Pure alkaline phosphatase, its preparation and use
The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg++ inhibitor: EDTA (iii) thermal stability: 45 DEG C. or below (iv) optimum temperature: 35 DEG -45 DEG C. (v) pH stability: 6-11 (vi) pH optimum:...
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| creator | SENIOR STEPHANIE J MAY KEITH GANI MOHAMED M |
| description | The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg++ inhibitor: EDTA (iii) thermal stability: 45 DEG C. or below (iv) optimum temperature: 35 DEG -45 DEG C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed. |
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(v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.</description><edition>4</edition><language>eng</language><subject>BEER ; BIOCHEMISTRY ; CHEMISTRY ; COMPOSITIONS OR TEST PAPERS THEREFOR ; COMPOSITIONS THEREOF ; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ; CULTURE MEDIA ; ENZYMOLOGY ; FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE ; INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES ; MEASURING ; MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS ; METALLURGY ; MICROBIOLOGY ; MICROORGANISMS OR ENZYMES ; MUTATION OR GENETIC ENGINEERING ; ORGANIC CHEMISTRY ; PEPTIDES ; PHYSICS ; PROCESSES OF PREPARING SUCH COMPOSITIONS ; PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS ; SPIRITS ; TESTING ; VINEGAR ; WINE</subject><creationdate>1987</creationdate><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19870421&DB=EPODOC&CC=US&NR=4659666A$$EHTML$$P50$$Gepo$$Hfree_for_read</linktohtml><link.rule.ids>230,308,776,881,25542,76289</link.rule.ids><linktorsrc>$$Uhttps://worldwide.espacenet.com/publicationDetails/biblio?FT=D&date=19870421&DB=EPODOC&CC=US&NR=4659666A$$EView_record_in_European_Patent_Office$$FView_record_in_$$GEuropean_Patent_Office$$Hfree_for_read</linktorsrc></links><search><creatorcontrib>SENIOR; STEPHANIE J</creatorcontrib><creatorcontrib>MAY; KEITH</creatorcontrib><creatorcontrib>GANI; MOHAMED M</creatorcontrib><title>Pure alkaline phosphatase, its preparation and use</title><description>The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg++ inhibitor: EDTA (iii) thermal stability: 45 DEG C. or below (iv) optimum temperature: 35 DEG -45 DEG C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.</description><subject>BEER</subject><subject>BIOCHEMISTRY</subject><subject>CHEMISTRY</subject><subject>COMPOSITIONS OR TEST PAPERS THEREFOR</subject><subject>COMPOSITIONS THEREOF</subject><subject>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</subject><subject>CULTURE MEDIA</subject><subject>ENZYMOLOGY</subject><subject>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</subject><subject>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</subject><subject>MEASURING</subject><subject>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</subject><subject>METALLURGY</subject><subject>MICROBIOLOGY</subject><subject>MICROORGANISMS OR ENZYMES</subject><subject>MUTATION OR GENETIC ENGINEERING</subject><subject>ORGANIC CHEMISTRY</subject><subject>PEPTIDES</subject><subject>PHYSICS</subject><subject>PROCESSES OF PREPARING SUCH COMPOSITIONS</subject><subject>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</subject><subject>SPIRITS</subject><subject>TESTING</subject><subject>VINEGAR</subject><subject>WINE</subject><fulltext>true</fulltext><rsrctype>patent</rsrctype><creationdate>1987</creationdate><recordtype>patent</recordtype><sourceid>EVB</sourceid><recordid>eNrjZDAKKC1KVUjMyU7MycxLVSjIyC8uyEgsSSxO1VHILClWKChKLUgsSizJzM9TSMxLUSgtTuVhYE1LzClO5YXS3Azybq4hzh66qQX58anFBYnJqXmpJfGhwSZmppZmZmaOxoRVAAAheyt1</recordid><startdate>19870421</startdate><enddate>19870421</enddate><creator>SENIOR; STEPHANIE J</creator><creator>MAY; KEITH</creator><creator>GANI; MOHAMED M</creator><scope>EVB</scope></search><sort><creationdate>19870421</creationdate><title>Pure alkaline phosphatase, its preparation and use</title><author>SENIOR; STEPHANIE J ; MAY; KEITH ; GANI; MOHAMED M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-epo_espacenet_US4659666A3</frbrgroupid><rsrctype>patents</rsrctype><prefilter>patents</prefilter><language>eng</language><creationdate>1987</creationdate><topic>BEER</topic><topic>BIOCHEMISTRY</topic><topic>CHEMISTRY</topic><topic>COMPOSITIONS OR TEST PAPERS THEREFOR</topic><topic>COMPOSITIONS THEREOF</topic><topic>CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES</topic><topic>CULTURE MEDIA</topic><topic>ENZYMOLOGY</topic><topic>FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE</topic><topic>INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES</topic><topic>MEASURING</topic><topic>MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS</topic><topic>METALLURGY</topic><topic>MICROBIOLOGY</topic><topic>MICROORGANISMS OR ENZYMES</topic><topic>MUTATION OR GENETIC ENGINEERING</topic><topic>ORGANIC CHEMISTRY</topic><topic>PEPTIDES</topic><topic>PHYSICS</topic><topic>PROCESSES OF PREPARING SUCH COMPOSITIONS</topic><topic>PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS</topic><topic>SPIRITS</topic><topic>TESTING</topic><topic>VINEGAR</topic><topic>WINE</topic><toplevel>online_resources</toplevel><creatorcontrib>SENIOR; STEPHANIE J</creatorcontrib><creatorcontrib>MAY; KEITH</creatorcontrib><creatorcontrib>GANI; MOHAMED M</creatorcontrib><collection>esp@cenet</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext_linktorsrc</fulltext></delivery><addata><au>SENIOR; STEPHANIE J</au><au>MAY; KEITH</au><au>GANI; MOHAMED M</au><format>patent</format><genre>patent</genre><ristype>GEN</ristype><title>Pure alkaline phosphatase, its preparation and use</title><date>1987-04-21</date><risdate>1987</risdate><abstract>The enzyme alkaline phosphatase having the following properties: (i) molecular weight: approximately 80 000 with 2 subunits of 40 000 (iii) activator: Mg++ inhibitor: EDTA (iii) thermal stability: 45 DEG C. or below (iv) optimum temperature: 35 DEG -45 DEG C. (v) pH stability: 6-11 (vi) pH optimum: 10 (vii) specific activity: 3000-5000 units/mg (viii) isoelectric range: between pH 5.0 and 6.0. An antibody-enzyme conjugate process for purifying alkaline phosphatase and a reagent test kit using the purified alkaline phosphatase are also disclosed.</abstract><edition>4</edition><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE |
| title | Pure alkaline phosphatase, its preparation and use |
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