Method and device for performing microoperations on cells

PCT No. PCT/SU83/00023 Sec. 371 Date Feb. 12, 1985 Sec. 102(e) Date Feb. 12, 1985 PCT Filed Jun. 30, 1983 PCT Pub. No. WO85/00314 PCT Pub. Date Jan. 31, 1985.A method for performing microoperations on cells consists in that a cell is secured to a micro suction cup and the cell membrane is perforated...

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Hauptverfasser: FIKHTE, BORIS A, NIKITIN, VLADIMIR A, KHOKHLOV, ANATOLY M, LARIN, VYACHESLAV T
Format: Patent
Sprache:eng
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Zusammenfassung:PCT No. PCT/SU83/00023 Sec. 371 Date Feb. 12, 1985 Sec. 102(e) Date Feb. 12, 1985 PCT Filed Jun. 30, 1983 PCT Pub. No. WO85/00314 PCT Pub. Date Jan. 31, 1985.A method for performing microoperations on cells consists in that a cell is secured to a micro suction cup and the cell membrane is perforated by a microinstrument. The cell membrane is pierced in a spot under the micro suction cup, the microinstrument being introduced through the recess of the micro suction cup, which is filled with an isotonic solution. A device for performing microoperations on cells comprises a microinstrument (1) installed in a holder (2) rigidly secured to a movable tip (3) a three-way micromanipulator (4), and a micro suction cup (5). The latter is rigidly secured to the holder (2), the microinstrument (1) is located in the recess of the micro suction cup (5) coaxially therewith and is connected to the holder (2) by a means (7a) ensuring the reciprocating movement of the microinstrument (1) along the axis of the micro suction cup (5).