Method for preparation of pure hepatitis B surface antigen from human plasma

Method for preparation of pure hepatitis B surface antigen HBsAg from human plasma, wherein the initial plasma after being treated to remove lipides and partially remove human plasma proteins, is submitted to digestion with pepsin in amount of 0.01-0.50 mg/mg of protein to selectively decompose the...

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Hauptverfasser:	DABROWA, ANDRZEJ, MADALINSKI, KAZIMIERZ, JANICKI, PIOTR, BRZOSKO, WITOLD, LASKOWSKI, ZBIGNIEW
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS ORMEDICINAL PREPARATIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Method for preparation of pure hepatitis B surface antigen HBsAg from human plasma, wherein the initial plasma after being treated to remove lipides and partially remove human plasma proteins, is submitted to digestion with pepsin in amount of 0.01-0.50 mg/mg of protein to selectively decompose the plasma proteins while the antigen remains intact, filtered in a phosphate buffered medium, up to a pH value of 7.1-7.3, through molecular filters passing molecules of up to 100,000 Daltons, the infectiosity being subsequently de-activated by means of formaldehyde, in proportion of 1:2000 by volume, for 96 hours.