Radioenzymatic method for assaying normetanephrine

Normetanephrine, an N-methylatable phenylethanolamine, is converted to its N-methylated, tritiated derivative, metanephrine, utilizing phenylethanolamine-N-methyltransferase as enzyme and tritiated S-adenosyl-L-methionine as methyl donor. The formed tritiated derivative is selectively extracted in o...

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Bibliographische Detailangaben
Hauptverfasser:	VLACHAKIS, NICOLAS D
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Normetanephrine, an N-methylatable phenylethanolamine, is converted to its N-methylated, tritiated derivative, metanephrine, utilizing phenylethanolamine-N-methyltransferase as enzyme and tritiated S-adenosyl-L-methionine as methyl donor. The formed tritiated derivative is selectively extracted in organic solvents and separated from other N-methylatable phenylethanolamines by thin layer chromatography. This assay could be used for measurement of normetanephrine in biological systems of patients with hypertension for detection of pheochromocytoma.