Assaying of digoxin by means of bioluminescence and inhibition of NA+ - K+-ATPase

A method is described for quantitative measurement of digoxin and other digitaloids in serum. The method is based on the inhibition of Na+, K+-specific ATPase enzyme by digitaloids as a result of receptor binding principle and the measurement of this inhibition with the firefly luceferin-luciferase...

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Hauptverfasser:	TARRKKANEN, VEIKKO, KOLEHMAINEN, SEPPO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method is described for quantitative measurement of digoxin and other digitaloids in serum. The method is based on the inhibition of Na+, K+-specific ATPase enzyme by digitaloids as a result of receptor binding principle and the measurement of this inhibition with the firefly luceferin-luciferase bioluminescence assay of APT. Method is specific and has similar sensitivity to that of the commonly used radioimmunoassay, but simpler and can be performed with low-cost instrumentation.