Tris (hydroxymethyl) aminomethane salt of 2-mercaptosuccinic acid

An improved reagent system for chemical determinations and novel compositions therefor provide improved reagent stability, particularly in a dry, storage stable phase. For determinations in which hexokinase and glucose-6-phosphate dehydrogenase are indicator enzymes, glycine and taurine are combined...

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Bibliographische Detailangaben
Hauptverfasser:	GREEN, STANLEY E
Format:	Patent
Sprache:	eng
Schlagworte:	ACYCLIC OR CARBOCYCLIC COMPOUNDS BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	An improved reagent system for chemical determinations and novel compositions therefor provide improved reagent stability, particularly in a dry, storage stable phase. For determinations in which hexokinase and glucose-6-phosphate dehydrogenase are indicator enzymes, glycine and taurine are combined with bovine serum albumin to provide a bulking agent for the indicator enzymes which also acts as a stabilizer. A novel salt, the tris (hydroxymethyl)aminomethane salt of 2 mercaptosuccinic acid, acts as an activator or stabilizer in chemistries utilizing the indicator enzymes, namely determinations of creatine phosphokinase or glucose. The novel salt also acts as a stabilizer in a urea nitrogen determination. Dry reagent components are bulked in triethanolammonium terepthalate. Novel CPK, glucose and serum urea nitogen determinations are also provided.