Continuous cell culture system

A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	FEDER, JOSEPH, TOLBERT, WILLIAM R, KIMES, RICHARD C
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.