Separation of active alpha 1-acid glycoprotein and utilization in the lipoprotein lipase enzyme system

This invention relates to a method of separating the active alpha 1-acid glycoprotein fraction, a co-factor in the lipoprotein lipase reaction, from urine of nephrotic animals and humans as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 w...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Hauptverfasser:	FELTS, JAMES M, STAPRANS, ILONA
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS HUMAN NECESSITIES HYGIENE MEDICAL OR VETERINARY SCIENCE METALLURGY ORGANIC CHEMISTRY PEPTIDES PREPARATIONS FOR MEDICAL, DENTAL, OR TOILET PURPOSES TECHNICAL SUBJECTS COVERED BY FORMER USPC TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ARTCOLLECTIONS [XRACs] AND DIGESTS
Online-Zugang:	Volltext bestellen
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	This invention relates to a method of separating the active alpha 1-acid glycoprotein fraction, a co-factor in the lipoprotein lipase reaction, from urine of nephrotic animals and humans as follows: (a) concentrate the said urine to about 10-20% by volume; (b) precipitate undesired protein at pH 4 with ammonium sulfate; and (c) recover the alpha 1-acid glycoprotein fraction from the supernatant, neutralize with solid NaHCO3, and purify by dialysis versus H2O and lyophilize. This active alpha 1-acid glycoprotein fraction is utilized in effective amounts in nephrotic animals to reverse the defect in triglyceride removal caused by the loss of plasma constituents in urine.