Method for the determination of hemoglobin in trace amounts
An improved technique for determining the presence of hemoglobin, having increased sensitivity, is provided by bringing the material to be tested into contact with an acidic solution of tetramethylbenzidine and hydrogen peroxide to form a mixture, allowing said mixture to stand for a predetermined p...
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| Format: | Patent |
| Sprache: | eng |
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| Zusammenfassung: | An improved technique for determining the presence of hemoglobin, having increased sensitivity, is provided by bringing the material to be tested into contact with an acidic solution of tetramethylbenzidine and hydrogen peroxide to form a mixture, allowing said mixture to stand for a predetermined period of time, and thereafter examining the mixture for the presence of a colored complex indicative of the presence of hemoglobin. Organic acid is present in a concentration generally of at least 30 percent by volume, to provide a pH of no more than about 2. |
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