Process for the preservation of ram semen by freezing

The process according to the invention consists in that the freshly collected ram semen is first diluted at between 25 DEG and 32 DEG C and notably at 30 DEG C, in a diluent without glycerol containing lactose and egg yolk, and in then diluting, at between 3 DEG and 5 DEG C and notably at 4 DEG C, t...

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Hauptverfasser: COLAS, GUY
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The process according to the invention consists in that the freshly collected ram semen is first diluted at between 25 DEG and 32 DEG C and notably at 30 DEG C, in a diluent without glycerol containing lactose and egg yolk, and in then diluting, at between 3 DEG and 5 DEG C and notably at 4 DEG C, the mixture previously obtained in a diluent containing glycerol, said diluent essentially comprising powdered milk and sodium citrate, in conditioning in straws the so diluted semen and in freezing it in liquid nitrogen at about -75 DEG C. The invention also relates to the diluent used in the above process.