PROCESS FOR PRODUCING URICASE

Uricase is produced by incubating Corynebacterium uratoxidans nov.sp. U-23 (ATCC No. 21749), U-8 (ATCC No. 21750), U-30 (ATCC No. 21751) or U-125 (ATCC No. 21752) in a medium containing 0.01 to 1.0% (W/V) of uric acid and preferably 0.1 to 3.0% (V/V) of at least one of alcohols having 1 to 8 carbons...

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Bibliographische Detailangaben
Hauptverfasser:	SUGISAKI Z,JA, WATANABE N,JA, IGUCHI N,JA, KOYAMA H,JA, KOBAYASHI T,JA
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Uricase is produced by incubating Corynebacterium uratoxidans nov.sp. U-23 (ATCC No. 21749), U-8 (ATCC No. 21750), U-30 (ATCC No. 21751) or U-125 (ATCC No. 21752) in a medium containing 0.01 to 1.0% (W/V) of uric acid and preferably 0.1 to 3.0% (V/V) of at least one of alcohols having 1 to 8 carbons at a pH of 5.5 to 9.0 and a temperature of 20 DEG to 40 DEG C under aerobic conditions, thereby producing and accumulating the extracellular uricase in the surrounding medium and recovering the same from the cultured broth. The uricase is useful as a reagent for biochemical diagnosis.