METHOD AND APPARATUS FOR THE PRESERVATION OF BIOLOGICAL SUBSTANCES
Biological substances such as blood are stored under pressure at below 0 DEG C temperatures, in a substantially non-dilatable vessel. Upon being introduced into the vessel, the temperature of the vessel and the blood are reduced to 4 DEG C; when equilibrium has been achieved the blood within the ves...
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Zusammenfassung: | Biological substances such as blood are stored under pressure at below 0 DEG C temperatures, in a substantially non-dilatable vessel. Upon being introduced into the vessel, the temperature of the vessel and the blood are reduced to 4 DEG C; when equilibrium has been achieved the blood within the vessel is precompressed at a controlled rate several thousand pounds per square inch by the application of mechanical leverage to a vessel piston. Thereafter, the vessel and blood are reduced to a final temperature from 0 DEG C to -21 DEG C. This temperature reduction, because of the conjunctive effect of the volumetric expansion of the blood and non-dilation of the vessel, self-generates a pressure from 15 to 35,000 p.s.i. The vessel may now be stored at the final temperature and the blood within it maintained without deleterious effects. |
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