Process for preparing a biochemically active polypeptide from sheep pituitary growthhormone

The invention comprises an active polypeptide composition characterized by being electrophoretically homogeneous, having a molecular weight between 6600 and 8000, producing growth in stabilized adult female rats, producing a tibial epiphyseal response in hypophysectomized female rats, accelerating t...

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Bibliographische Detailangaben
1. Verfasser: BORNSTEIN JOSEPH
Format: Patent
Sprache:eng
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Zusammenfassung:The invention comprises an active polypeptide composition characterized by being electrophoretically homogeneous, having a molecular weight between 6600 and 8000, producing growth in stabilized adult female rats, producing a tibial epiphyseal response in hypophysectomized female rats, accelerating the utilization of glucose by muscle, accelerating the oxidation of C14 glucose by adipose tissue located in the intestinal section and also accelerating the rate of C14 amino acid incorporation into the protein of muscle and into the protein of epididymal adipose tissue. The polypeptide is prepared from purified preparations of animal or human growth hormones or from impure preparations containing these, e.g. animal pituitary glands or the acetone-insoluble portion of animal pituitary glands. The growth hormone preparation is subjected to acid hydrolysis at a pH of 1.5 to 2.5 preferably in an aqueous medium containing ethanol, n-butanol, toluene and water in the proportions of 4 : 0.4 : 0.4 : 1 by volume. The insoluble portion is recovered from the extraction solvent and dried, extracted with 0.1 M acetic acid, and the extract so formed is filtered through an ultrafilter. The ultrafiltrate is passed through a cation-exchange resin on the hydrogen cycle, washed with water to recover unabsorbed material, and the aqueous solution treated with an ethanol-ether mixture to precipitate the active polypeptide. The dried precipitate is dissolved in water, the pH adjusted to 8.5 with ammonia, the resultant solution filtered and the polypeptide precipitated by the addition of a mixture of ethanol and ether.