Microinjection system and method

A microinjection system (1) for microinjection of biological samples, comprising a sample holder (2) for supporting a biological sample (S); a first camera assembly (3) spaced apart from the sample holder (2); a needle holder (4) moveably arranged above the sample holder (2). The needle holder (4) is configured to removably receive a hollow microneedle (5), the first camera assembly (3) being configured for imaging the biological sample (S) along an optical axis (O). The needle holder (4) and sample holder (2) are spaced apart at a variable distance along the optical axis (O) for moving a tip part (6) of the microneedle (5) towards or away from the biological sample (S). The sample holder (2) is moveable in a plane (P) perpendicular to the optical axis (O). The needle holder (4) is rotatable around the optical axis (O) for rotating the microneedle (5) around the optical axis (O).