GRAPHENE ENERGY TRANSFER WITH VERTICAL NUCLEIC ACIDS

GRAPHENE ENERGY TRANSFER WITH VERTICAL NUCLEIC ACIDS

A construct comprising a graphene substrate and a hybrid molecule comprising a single-stranded nucleic acid segment and a double-stranded nucleic acid segment, in which the hybrid molecule is immobilized to the graphene substrate by the single stranded nucleic acid segment, and in which the double-s...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SZALAI, Alan M, FERRARI, Giovanni, RICHTER, Lars, KAMINSKA, Izabela, TINNEFELD, Philip
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
TESTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A construct comprising a graphene substrate and a hybrid molecule comprising a single-stranded nucleic acid segment and a double-stranded nucleic acid segment, in which the hybrid molecule is immobilized to the graphene substrate by the single stranded nucleic acid segment, and in which the double-stranded nucleic acid segment comprises a linear segment comprising at least one base pair in perpendicular orientation to the graphene substrate at the junction where the double-stranded nucleic acid segment and the single-stranded nucleic acid segment join. In addition, a method for producing such a construct, the construct as produced by the production method of the invention, a method for measuring quenching efficiency by means of measuring the fluorescence lifetime or fluorescence intensity in such a construct, as well as a microscopy system for measuring relative fluorescence lifetime in such a construct.