METHODS AND SYSTEMS FOR SORTING BIOLOGICAL PARTICLES

The present disclosure provides methods and systems for separating one or more target analytes from a fluid sample. The systems may comprise a microfluidic device. The microfluidic device may comprise a fluidic channel having an array of obstacles disposed therein. The array of obstacles may be oriented at an angle greater than 0° relative to a direction of a fluid flow in the fluidic channel. The array of obstacles may be configured to separate the target analytes from the fluid upon flow of the fluid through the fluidic channel. The methods of the present disclosure may comprise separating target analytes from a fluid using a microfluidic device comprising obstacles disposed in a fluidic channel of the device. The target analytes may be separated with a high efficiency, sensitivity and/or specificity. Also disclosed herein are devices, methods, and systems for separating one or more biological particles from a fluid sample. The devices may comprise a substrate with a fluidic channel disposed therein. The fluidic channel has disposed therein an array of obstacles with a vertical spacing. The vertical spacing may be configured to separate one or more particles from a fluid stream when the stream flows through the fluidic channel. The devices, methods, and systems may be able to separate various types of biological particles at a high efficiency, sensitivity, and/or specificity.