APPARATUSES, METHODS, AND KITS FOR MICROFLUIDIC ASSAYS

APPARATUSES, METHODS, AND KITS FOR MICROFLUIDIC ASSAYS

Methods, systems, and kits for determining a level of dissolved oxygen within a microfluidic device are provided. The microfluidic device can be suitable for cell culture. The methods, systems, and kits can further be used to determine a level of oxygen consumption in a population of biological micr...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: LOWE, JR., Randall D, MASTROIANNI, Alexander J, HUBBARD, Jonathan Cloud Dragon, SACKMANN, Eric K, LIONBERGER, Troy A, CHIEN, Alexander, LIN, Ke-Chih, INGRAM, Patrick N, GADISH, Or, KURZ, Volker L.S
Format: Patent
Sprache:eng
Schlagworte:
APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PHYSICS
SPIRITS
TESTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Methods, systems, and kits for determining a level of dissolved oxygen within a microfluidic device are provided. The microfluidic device can be suitable for cell culture. The methods, systems, and kits can further be used to determine a level of oxygen consumption in a population of biological micro-objects. In particular, the methods, systems, and kits of the present disclosure rely on flowing a fluidic medium containing a dye and a supplied partial pressure of oxygen into the microfluidic device for a period of time, wherein fluorescence emitted by the dye changes based on availability of oxygen proximate to the dye; taking a fluorescence image of an area of interest within the chamber; and correlating fluorescence of the fluorescence image of the area of interest to a reference to determine an observed partial pressure of the oxygen in the area of interest.