NUCLEIC ACID CAPTURE, CONCENTRATION, AND PURIFICATION

An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chambe...

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Bibliographische Detailangaben
Hauptverfasser: Prabhu, Anmiv, Higgins, Michelle, Pantoja, Rigo, Ramirez, Sean M
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:An example of a kit includes a flow cell assembly. The flow cell assembly includes a reaction chamber, a temperature controlled flow channel in selective fluid communication with an inlet of the reaction chamber, and a filter positioned in the temperature controlled flow channel. The reaction chamber includes depressions separated by interstitial regions and capture primers attached within each of the depressions. The filter is i) to block concentrated biological sample-polymer complexes generated in the temperature controlled flow channel at a first temperature, and ii) to allow passage of concentrated biological sample and polymer released from the complexes in the temperature controlled flow channel at a second temperature.