POLYNUCLEOTIDE DETECTION

This invention relates to a method of detecting two or more target polynucleotide sequences comprising using a probe oligonucleotide A0 annealing to the target, whereafter the 3′ end is pyrophosphorolysed and the remaining probe is circularised or ligated to another probe using a splint oligonucleot...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	BALMFORTH, Barnaby William, STOLAREK-JANUSZKIEWICZ, Magdalena
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	This invention relates to a method of detecting two or more target polynucleotide sequences comprising using a probe oligonucleotide A0 annealing to the target, whereafter the 3′ end is pyrophosphorolysed and the remaining probe is circularised or ligated to another probe using a splint oligonucleotide, and a padlock probe X0, which is circularised against the target and not pyrophosphorolysed, and then a signal from (amplicons of) the ligated probes is detected to indicate presence of the target. Further disclosed are kits comprising the probes and enzymes as well as buffer and a source of pyrophosphate ions.