METHOD FOR USING TANGENTIAL FLOW ULTRAFILTRATION TECHNOLOGY TO PREPARE CONTROLLABLE HIGH CONCENTRATION SILK FIBROIN SOLUTION

A method for preparing a controllable high-concentration silk fibroin solution with high molecular weight using tangential flow ultrafiltration technology is provided. It includes steps of acquiring an inorganic salt solution of silk fibroin; subjecting the inorganic salt solution of silk fibroin to membrane clarification process; desalting the inorganic salt solution of silk fibroin by filtering the clarified solution obtained from the membrane clarification process through a tangential flow filtration system; and concentrating the silk fibroin solution to give a high-concentration aqueous solution of silk fibroin with high molecular weight. The method accomplishes the desalination and concentration of a salt solution of silk fibroin by the tangential flow filtration technology, and directly acquires an aqueous solution of silk fibroin with a number-average molecular weight of 80 kDa-200 kDa.