GENE ANALYSIS METHOD AND KIT FOR GENE ANALYSIS

Provided is a gene analysis method including performing a single-base extension reaction using a primer for a single-base extension reaction for detection of a target nucleotide sequence, and a substrate for a single-base extension reaction having a fluorescent dye; subjecting a reaction product of...

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Bibliographische Detailangaben
Hauptverfasser:	Manri, Chihiro, Yokoi, Takahide, Ishida, Takeshi, Ando, Takahiro
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Provided is a gene analysis method including performing a single-base extension reaction using a primer for a single-base extension reaction for detection of a target nucleotide sequence, and a substrate for a single-base extension reaction having a fluorescent dye; subjecting a reaction product of the single-base extension reaction to electrophoresis; and measuring mobility of the electrophoresis and fluorescence intensity of the fluorescent dye, and detecting a wild type and a mutant of the target nucleotide sequence based on the fluorescence intensity. Also provided is a kit for use in the gene analysis method disclosed herein.