Display Substrate, Manufacturing Method Thereof, and Display Device

The present disclosure provides a display substrate, a manufacturing method thereof and a display device. The display substrate includes: a substrate structure including a base substrate; and a light-emitting structure, a pixel defining layer and a connection structure wherein the light-emitting structure includes a conductive structure layer, a first light-emitting layer and a first electrode layer. The connection structure includes a first conductive layer, a second conductive layer and a top structure, wherein an orthographic projection of the second conductive layer on the base substrate is inside an orthographic projection of the top structure on the base substrate, and the top structure includes a second light-emitting layer, wherein an orthographic projection of the first light-emitting layer on the base substrate partially overlaps with an orthographic projection of the second light-emitting layer on the base substrate.