VECTOR MANUFACTURING PROCESSES

The present disclosure provides viral vector manufacturing and purification methods. Particularly, the disclosure provides improved methods for manufacturing lentiviral vector from host cells grown in suspension. More particularly, the disclosure provides improved large-scale lentiviral manufacturin...

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Bibliographische Detailangaben
Hauptverfasser:	Bhattacharya, Moumita, Kral, Kelly Marie, Kuczewski, Michael Christopher, Kapadia, Adnan, Kelly, Kristen
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SEPARATION SPIRITS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure provides viral vector manufacturing and purification methods. Particularly, the disclosure provides improved methods for manufacturing lentiviral vector from host cells grown in suspension. More particularly, the disclosure provides improved large-scale lentiviral manufacturing methods comprising growing cells to a suitable number, transfecting with lentiviral packaging plasmids, a transfer plasmid, and a transfection agent; treating with an endonuclease; harvesting and clarifying the suspension culture supernatant; capturing and concentrating the lentiviral vector using affinity chromatography or cation exchange chromatography; filtering the concentrated lentiviral vector; ultrafiltering and diafiltering the lentiviral vector; formulating the lentiviral vector; and sterile filtering the formulated bulk lentiviral vector.