SYSTEMS AND METHODS FOR CELL-FREE POLYPEPTIDE SYNTHESIS

Disclosed herein is a method for cell-free polypeptide synthesis. The method comprises lysing a cell to produce a cellular lysate, passing the cellular lysate through a filter to produce a cellular extract, contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a react...

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Bibliographische Detailangaben
Hauptverfasser:	Kightlinger, Weston Kevin, Shah-Morales, Sajita Caroni, Yoesep, Danielle Jessica
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed herein is a method for cell-free polypeptide synthesis. The method comprises lysing a cell to produce a cellular lysate, passing the cellular lysate through a filter to produce a cellular extract, contacting the cellular extract with a nucleic acid encoding a polypeptide to produce a reaction mixture, and incubating the reaction mixture to produce the polypeptide.