NON-ANIMAL CHONDROITIN SULFATE OLIGOSACCHARIDE AND PREPARATION METHOD THEREOF

The invention provides a non-animal chondroitin sulfate oligosaccharide and a preparation method thereof. The method includes a one-step chemical procedure and an enzyme catalysis procedure that are orderly carried out, including: extracting Escherichia coli K4 polysaccharide, chemically removing th...

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Bibliographische Detailangaben
Hauptverfasser:	XU, Shuqin, WANG, Zhuqun, CHEN, Jinghua
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention provides a non-animal chondroitin sulfate oligosaccharide and a preparation method thereof. The method includes a one-step chemical procedure and an enzyme catalysis procedure that are orderly carried out, including: extracting Escherichia coli K4 polysaccharide, chemically removing the fructosyl group therefrom, and degrading with a chondroitin sulfate degrading enzyme to obtain a chondroitin oligosaccharide mixture; preparing chondroitin disaccharide to octasaccharide by separation by separation by a centrifugal ultrafiltration tube, Bio-Gel P-2 gel exclusion chromatography, and high performance liquid chromatography (HPLC); and enzymatically modifying the obtained products by 4-O-sulfation and 6-O-sulfation, to obtain chondroitin sulfate CS-A and CS-C oligosaccharides respectively. The raw materials are from non-animal sources, the pollution risk is low, the reaction conditions are mild and efficient, and the structures and molecular weights of the prepared chondroitin sulfate disaccharide to octasaccharide are definite, providing possibility for the research of chondroitin sulfate oligosaccharides with single degrees of polymerization.