XYLOSYLATED STEVIOL GLYCOSIDES AND ENZYMATIC METHODS FOR PRODUCTION

XYLOSYLATED STEVIOL GLYCOSIDES AND ENZYMATIC METHODS FOR PRODUCTION

Disclosed are methods and compositions for producing xylosylated steviol glycosides that include a xylose residue, or an oligosaccharide moiety including a xylose residue, attached to the 19 carbon of the steviol base. A glycosyltransferase having UDP-xylose:19-steviol xylosyltransferase activity (s...

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Bibliographische Detailangaben
Hauptverfasser: GASPARD, Daniel Scott, MARASCO, Erin Kathleen, KYRIAKOU, Panagiota, EVANS, Erin, ZHENG, Guo-Hua
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Disclosed are methods and compositions for producing xylosylated steviol glycosides that include a xylose residue, or an oligosaccharide moiety including a xylose residue, attached to the 19 carbon of the steviol base. A glycosyltransferase having UDP-xylose:19-steviol xylosyltransferase activity (such as one of SEQ ID NO:1 or a homolog or variant thereof) forms the xylosylated steviol glycoside using a steviol glycoside acceptor having a glucose residue attached to the 19 carbon of the steviol base. Glycosyltransferase variants with increased activity are also described.