METHOD OF PRODUCING HAIRPIN SINGLE-STRANDED RNA MOLECULE

METHOD OF PRODUCING HAIRPIN SINGLE-STRANDED RNA MOLECULE

A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, the method including: (i) an annealing step of annealing a first single-stranded oligoRNA molecule and a second single-stranded oligoRNA molecule; and (ii) a ligation step of ligating 3′ end o...

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Hauptverfasser: Inada, Hideaki, Takashina, Ayumi, Iseki, Katsuhiko, Okimura, Keiichi, Sanosaka, Masato
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method produces a hairpin single-stranded RNA molecule capable of inhibiting expression of a target gene, the method including: (i) an annealing step of annealing a first single-stranded oligoRNA molecule and a second single-stranded oligoRNA molecule; and (ii) a ligation step of ligating 3′ end of the first single-stranded oligoRNA molecule and 5′ end of the second single-stranded oligoRNA molecule by an Rn12 family ligase, wherein a sequence produced by ligating the first single-stranded oligoRNA molecule and the second single-stranded oligoRNA molecule includes a gene expression-inhibiting sequence for the target gene.