CAPILLARY ELECTROPHORESIS METHODS FOR QUANTIFYING VIRAL SPECIES
Methods and kits for quantifying a viral species are presently claimed and described. The method includes the steps of preparing at least one labeled viral protein component by incubating a detectable dye with a virial species in the presence of sodium dodecyl sulfate (SDS); loading the labeled vira...
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Zusammenfassung: | Methods and kits for quantifying a viral species are presently claimed and described. The method includes the steps of preparing at least one labeled viral protein component by incubating a detectable dye with a virial species in the presence of sodium dodecyl sulfate (SDS); loading the labeled viral protein component onto a capillary electrophoresis (CE) capillary, wherein the CE capillary is filled with a buffer comprising a polymer matrix; applying a separation voltage to the CE capillary; detecting at least one labeled viral protein component with a detector, thereby producing a corresponding set of values; and quantifying a protein of interest in the viral protein component using the corresponding set of values. |
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