PREPARATION METHOD OF SYNTHETASE FOR CONTINUOUS MONITORING OF BLOOD GLUCOSE

The present invention discloses a preparation method of a synthetase for continuous monitoring of blood glucose. The method includes the preparation steps of step S1: hydroxylation of a glass bead; step S2: silanization of the glass bead; step S3: bonding of the glass bead to glucose; step S4: synth...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Hauptverfasser: ZHANG, Shaoda, Zheng, Xingyu, Qiu, Zhipeng
Format: Patent
Sprache:eng
Schlagworte:
Online-Zugang:Volltext bestellen
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The present invention discloses a preparation method of a synthetase for continuous monitoring of blood glucose. The method includes the preparation steps of step S1: hydroxylation of a glass bead; step S2: silanization of the glass bead; step S3: bonding of the glass bead to glucose; step S4: synthesis of a polymer particle on the surface of the glass bead to form a glucose synthetase; and step S5: separation of the highly specific glucose synthetase. According to the preparation method of a synthetase for continuous monitoring of blood glucose provided by the present invention, the glass bead is bonded to the glucose by activating the surface of the glass bead, and then bonded to a polymer to form the glucose synthetase. The artificial synthetase prepared by the method has low sensitivity to temperature and pH value, and can be directly used in a blood glucose electrochemical sensor.