Method for Producing Human Chimeric Antigen Receptor T-Cell Population Enriched with Stem Cell-like Memory T-Cells

The present invention provides a method for producing CAR T-cells enriched with stem cell-like memory T-cells (Tscm), comprised of: (1) providing PBMCs; (2) isolating of T cells from PBMCs using magnetic beads coated with T-cell specific antibodies; (3) positively selecting T cells by culturing them in the form of T cell-magnetic bead complexes; (4) adding CAR-expressing lentivirus to the activated human T cell-magnetic bead complexes, for transduction of human T cells into CAR-T cells on the human T cell-magnetic bead complexes to obtain human CAR-T cell-magnetic bead complexes; (5) obtaining human CAR-T cells by dissociating the human CAR-T cells from the human CAR-T cell-magnetic bead complexes; and (6) further culturing CAR-T cells to obtain CAR-T cells enriched with Tscm. The present invention expands T cells in a short period of time to obtain CAR-T cells enriched with Tscm, which is suitable for CAR-T immunotherapy.