METHOD OF DETECTING MULTIPLE FORMS OF AN ANALYTE

A method of determining whether one or more forms of a nucleic acid analyte are present in a sample. The method includes dissolving an amplification reagent with a first solvent, where the amplification reagent contains oligonucleotides sufficient to amplify and detect a first region of a first form of the analyte, where the first solvent contains one or more oligonucleotides which, in combination with the oligonucleotides of the amplification reagent, are sufficient to amplify and detect a second region of a second form of the analyte, where the one or more oligonucleotides of the first solvent are insufficient to amplify and detect the first or second form of the analyte, and where the first and second regions each comprise a different nucleotide base sequence. A purified form of the sample is contacted with the dissolved amplification reagent, thereby forming an amplification reaction mixture which is exposed to temperature conditions sufficient for amplifying the first and second regions of the first and second forms of the analyte, respectively. It is then determined whether the first and/or second forms of the analyte are present in the sample.