OLIGONUCLEOTIDE-TETHERED NUCLEOTIDES

The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligon...

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Hauptverfasser: Zeimyte, Simona, Sulgaite, Justina, Chen, Sihong, Lubys, Arvydas, Kapustina, Zana, Allen, Michael, Cikotiene, Inga, Berezniakovas, Arturas, Andersen, Mark
Format: Patent
Sprache:eng
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Zusammenfassung:The present disclosure describes oligonucleotide-tethered nucleotides, methods of making them, and methods of using them. The oligonucleotide-tethered nucleotides comprise, in some embodiments, a nucleotide linked to an oligonucleotide of from about 3 to about 100 nucleotides in length. These oligonucleotide-tethered nucleotides can be used to label a plurality of different types of nucleic acids in a plurality of different situations with a known oligonucleotide, which can serve as a barcode in some embodiments. The resulting oligonucleotide-labeled nucleic acids oligo-nucleotides can be used in a variety of nucleic acid sequencing methods.