Process for Controlled Manufacturing of Mono-Disperse Microbubbles

The present invention relates to a process for preparing a hydrated phospholipids solvent mixture, by: -dissolving a first phospholipid at a temperature above the phase transition temperature of the phospholipids in an organic solvent to form a dissolved phospholipid solvent mixture; -dissolving a second phospholipid at a temperature above the phase transition temperature of the phospholipids in the dissolved phospholipid solvent mixture to form a dissolved phospholipids solvent mixture; -adding an aqueous phosphate buffer to the dissolved phospholipids solvent mixture to form a buffered phospholipids solvent mixture; and -stirring the buffered phospholipids solvent mixture to form a hydrated phospholipids solvent mixture.