HIV Pseudovirus Particles and Method for Preparing the Same

HIV pseudovirus particles and method for preparing the same. The pseudovirus particles contain conserved sequences of gag, pol and LTR regions on genes from HIV-1 and a protein from phage MS2. The method comprises the following steps: designing and selecting target fragments and a sequence of gene from phage MS2; direct synthesizing both of them respectively; inserting the sequence of gene from phage MS2 into pETDuet-1 vector to obtain pETDuet-MS2 vector; inserting the target fragments into pETDuet-MS2 vector to obtain pETDuet-MS2-HIV recombinant expression vector. The sequence of gene from phage MS2 and target fragments respectively are located between any restriction sites behind the 3′ends of two T7 promoters of pseudovirus particles. The pseudovirus particles are encapsulated with a plurality of RNA conserved fragments from HIV-1 simultaneously, and have high yield and good stability. The method is fast, convenient, efficient and cost-effective.