METHODS FOR ORGANOID PASSAGING USING MICROPLATE WELL UNITS

Disclosed are various embodiments related to automated cellular passaging of spheroids, tumoroids, organoids and/or other multi-cellular bodies. Organoids can be cultured in a hydrogel that is disposed in a well unit of a microwell plate. The well unit includes a primary well section that is fluidly...

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Hauptverfasser:	SPIRA, Felix, ATZLER, Josef
Format:	Patent
Sprache:	eng
Schlagworte:	APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Disclosed are various embodiments related to automated cellular passaging of spheroids, tumoroids, organoids and/or other multi-cellular bodies. Organoids can be cultured in a hydrogel that is disposed in a well unit of a microwell plate. The well unit includes a primary well section that is fluidly connected to a secondary well section via at least one channel, and the hydrogel is disposed in the primary well section of the well unit. The hydrogel is dissipated into hydrogel fragments, thereby separating the organoids from the hydrogel. The hydrogel fragments are removed from the well unit, while the organoids remain in the well unit. The remaining organoids are broken into organoid fragments and corresponding debris. A fresh culture environment is created using the organoid fragments.