METHOD, COMPUTER PROGRAM, AND DATA PROCESSING UNIT FOR CREATING AT LEAST ONE CORRECTION VALUE FOR CORRECTING FLUORESCENCE INTENSITIES IN A FLUORESCENCE IMAGE, AND OPTICAL OBSERVATION SYSTEM

A method for creating at least one correction value for correcting fluorescence intensities in a fluorescence image obtained with an optical observation system includes determining the parameter value of at least one parameter of the optical observation system which influences the observation of the fluorescence intensity, and generating the at least one correction value for correcting the fluorescence intensities in the fluorescence image based on the determined parameter value and the influence of the at least one parameter which influences the observation of the fluorescence intensity on the fluorescence intensities. A parameter of the illumination system serves as the at least one parameter which influences the observation of the fluorescence intensity. Additionally, a computer program, a computer-implemented method, a data processing unit, and an optical observation system for creating at least one correction value for correcting fluorescence intensities in a fluorescence image are provided.