NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD
A nucleic acid amplification method is disclosed. The method employs a Bw adapter primer having a stem-loop structure to synthesize a complementary strand of a target region, followed by a further synthesis of a complementary strand employing an Fw adapter nucleotide that has a stem-loop structure a...
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| creator | NOTOMI, Tsugunori NAKAZATO, Hiroaki MORI, Yuta ASANO, Yoshinori |
| description | A nucleic acid amplification method is disclosed. The method employs a Bw adapter primer having a stem-loop structure to synthesize a complementary strand of a target region, followed by a further synthesis of a complementary strand employing an Fw adapter nucleotide that has a stem-loop structure and an extension-inhibiting modification at the 3′-end. The method makes it possible to simply and specifically form a dumbbell structure in which stem-loop structures are added to both ends of the complementary strand of the target region. |
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The method employs a Bw adapter primer having a stem-loop structure to synthesize a complementary strand of a target region, followed by a further synthesis of a complementary strand employing an Fw adapter nucleotide that has a stem-loop structure and an extension-inhibiting modification at the 3′-end. The method makes it possible to simply and specifically form a dumbbell structure in which stem-loop structures are added to both ends of the complementary strand of the target region.</abstract><oa>free_for_read</oa></addata></record> |
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| subjects | BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE |
| title | NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD |
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