NUCLEIC ACID AMPLIFICATION METHOD, PRIMER SET, PROBE, AND KIT FOR NUCLEIC ACID AMPLIFICATION METHOD

A nucleic acid amplification method is disclosed. The method employs a Bw adapter primer having a stem-loop structure to synthesize a complementary strand of a target region, followed by a further synthesis of a complementary strand employing an Fw adapter nucleotide that has a stem-loop structure a...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	NOTOMI, Tsugunori, NAKAZATO, Hiroaki, MORI, Yuta, ASANO, Yoshinori
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A nucleic acid amplification method is disclosed. The method employs a Bw adapter primer having a stem-loop structure to synthesize a complementary strand of a target region, followed by a further synthesis of a complementary strand employing an Fw adapter nucleotide that has a stem-loop structure and an extension-inhibiting modification at the 3′-end. The method makes it possible to simply and specifically form a dumbbell structure in which stem-loop structures are added to both ends of the complementary strand of the target region.