PLASMONIC NANOPARTICLE ASSISTED ENZYME-LINKED IMMUNOSORBENT ASSAY IN A FLUIDICS DEVICE

Methods for plasmonic nanoparticle assisted detection of target analytes are provided including methods of plasmonic nanoparticle assisted enzyme-linked immunosorbent assay (ELISA) in a fluidics device. For example, a digital microfluidics (DMF) system is provided that includes a DMF device (or cart...

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Bibliographische Detailangaben
Hauptverfasser:	DENOMME, Ryan Cameron, KRISHNAKUMAR, Harish, HUANG, Zhicheng, TAIAKINA, Valentina, LIU, Juewen
Format:	Patent
Sprache:	eng
Schlagworte:	INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MANUFACTURE OR TREATMENT OF NANOSTRUCTURES MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES MEASURING NANOTECHNOLOGY PERFORMING OPERATIONS PHYSICS SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES TESTING TRANSPORTING
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Beschreibung
Zusammenfassung:	Methods for plasmonic nanoparticle assisted detection of target analytes are provided including methods of plasmonic nanoparticle assisted enzyme-linked immunosorbent assay (ELISA) in a fluidics device. For example, a digital microfluidics (DMF) system is provided that includes a DMF device (or cartridge) in which the methods of plasmonic nanoparticle assisted ELISA may be performed. The disclosed methods for detecting target analytes include measuring an optically detectable change caused by one or a combination of etching, growth, aggregation, or altered interparticle distance of plasmonic particles in the vicinity of a target analyte-capture biomolecule complex in response to a product or byproduct generated by enzyme-substrate reactions. In the methods, the amount of enzyme-substrate reactions is proportional to the number of target analytes bound to the capture biomolecules.