STANDARDIZED AND VIRUS-FREE RAPID PROPAGATION METHOD OF DIANTHUS CARYOPHYLLUS
The present disclosure provides a standardized and virus-free rapid propagation method of Dianthus caryophyllus, belonging to the technical field of asexual propagation. The method includes the following steps: conducting virus detection on a Dianthus caryophyllus explant, and inoculating the explan...
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| creator | QU, Yunhui ZHANG, Yiping JIANG, Yalian YANG, Xiumei WANG, Jihua QU, Suping WANG, Lihua SU, Yan XU, Feng ZHAO, Axiang ZHANG, Lifang |
| description | The present disclosure provides a standardized and virus-free rapid propagation method of Dianthus caryophyllus, belonging to the technical field of asexual propagation. The method includes the following steps: conducting virus detection on a Dianthus caryophyllus explant, and inoculating the explant into an induction medium for induction culture; after the explant germinates, cutting buds, and determining whether a virus-free treatment is required according to a virus detection result, and continuing cultivating the buds; after cultivating to obtain cluster buds, inoculating the cluster buds into an expanding propagation medium for expanding propagation, and conducting the virus detection to eliminate a virus-carrying material; conducting expanding propagation on a virus-free material to a certain base, and transferring the virus-free material to a rooting medium for rooting culture. |
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| subjects | AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING |
| title | STANDARDIZED AND VIRUS-FREE RAPID PROPAGATION METHOD OF DIANTHUS CARYOPHYLLUS |
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