STANDARDIZED AND VIRUS-FREE RAPID PROPAGATION METHOD OF DIANTHUS CARYOPHYLLUS

The present disclosure provides a standardized and virus-free rapid propagation method of Dianthus caryophyllus, belonging to the technical field of asexual propagation. The method includes the following steps: conducting virus detection on a Dianthus caryophyllus explant, and inoculating the explan...

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Bibliographische Detailangaben
Hauptverfasser:	QU, Yunhui, ZHANG, Yiping, JIANG, Yalian, YANG, Xiumei, WANG, Jihua, QU, Suping, WANG, Lihua, SU, Yan, XU, Feng, ZHAO, Axiang, ZHANG, Lifang
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY FISHING FORESTRY HUMAN NECESSITIES HUNTING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES TRAPPING
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Beschreibung
Zusammenfassung:	The present disclosure provides a standardized and virus-free rapid propagation method of Dianthus caryophyllus, belonging to the technical field of asexual propagation. The method includes the following steps: conducting virus detection on a Dianthus caryophyllus explant, and inoculating the explant into an induction medium for induction culture; after the explant germinates, cutting buds, and determining whether a virus-free treatment is required according to a virus detection result, and continuing cultivating the buds; after cultivating to obtain cluster buds, inoculating the cluster buds into an expanding propagation medium for expanding propagation, and conducting the virus detection to eliminate a virus-carrying material; conducting expanding propagation on a virus-free material to a certain base, and transferring the virus-free material to a rooting medium for rooting culture.