GENE FOR SYNTHESIZING HIGH MOLECULAR WEIGHT COPOLYMER

The primary object of the present invention is to provide a polymer synthase gene which is derived from mangrove soil metagenome, and the method for producing the useful copolymer by using this polymer synthase. Another object of the present invention is to provide an enoyl-CoA hydratase gene which...

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Bibliographische Detailangaben
Hauptverfasser:	LAKSHMANAN, Manoj Kumar, C KANAPATHI PILLAI, K Sudesh Kumar, INOUE, Kozo, TAN, Hua Tiang
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The primary object of the present invention is to provide a polymer synthase gene which is derived from mangrove soil metagenome, and the method for producing the useful copolymer by using this polymer synthase. Another object of the present invention is to provide an enoyl-CoA hydratase gene which is derived from Streptomyces sp. CFMR 7, and the method for producing the useful copolymer, P(3HB-co-3HHx) with increasing the composition of 3HHx by the expression of this enoyl-CoA hydratase.In order to achieve these objects, an isolated polynucleotide encoding for a polypeptide with polymer synthase activity comprising an amino acid sequence set forth in SEQ ID NO: 1 or 3, or an amino acid sequence set forth in SEQ ID NO: 1 or 3 wherein one or more amino acids is replaced, deleted or added are provided.