GUIDE EXPRESSED AS AN EXTENSION OF AN MRNA

The present disclosure is directed to polynucleotides with a selection marker and a guide RNA module transcriptionally fused such that the selection marker and the genetic editing machinery are expressed concurrently. Accordingly, the selected cells are more likely to be genetically edited, such tha...

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Bibliographische Detailangaben
Hauptverfasser:	Moeller, Lorena Beatriz, Johnson, Ross Alastair, Dour, Scott, Soneji, Jaya, Vaghchhipawala, Zarir Erach, Moshiri, Farhad
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present disclosure is directed to polynucleotides with a selection marker and a guide RNA module transcriptionally fused such that the selection marker and the genetic editing machinery are expressed concurrently. Accordingly, the selected cells are more likely to be genetically edited, such that the genetic editing efficiency is increased. The present disclosure also provides a method of transforming plant cells, a method of altering a target site in the genome of a plant cell, a method of increasing the fraction of genetically edited cells in a plant embryo following transformation using the polynucleotides.