METHOD FOR MAGNETIC TRANSFECTION OF MAIZE POLLEN

The present invention discloses an improved method for magnetic transfection of maize pollen, including: (1) preparing plasmid DNA for transfection; (2) binding MNP nanomagnetic beads to the plasmid DNA at room temperature to form MNP-DNA complex; (3) collecting fresh pollen from maize at full flowering and bringing it rapidly back indoors under ice box storage conditions insulated from water; (4) mixing a maize pollen transformation solution with sieved maize pollen and being subjected to aperture-opening pre-treatment at low temperature; (5) adding the MNP-DNA complex to aperture-opening pre-treatment solution, mixing gently and placing on a low temperature pre-cooled magnetic plate for transfection; (6) after transfection, taking the pollen suspension to field in ice box and pollinating directly on ears of maize. The present invention directly uses maize grown in the field for transformation, requires only conventional refrigeration equipment, is simple and inexpensive to operate and is applicable to all maize varieties.