UDP-GLYCOSYLTRANSFERASES

The present disclosure relates a polypeptide having UGT activity, which polypeptide comprises an amino acid sequence which, when aligned with a polypeptide having UGT activity comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid corresponding to any o...

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Bibliographische Detailangaben
Hauptverfasser: BOER, Viktor Marius, VAN LEEUWEN, Johannes Gustaaf Ernst
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present disclosure relates a polypeptide having UGT activity, which polypeptide comprises an amino acid sequence which, when aligned with a polypeptide having UGT activity comprising the sequence set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid corresponding to any of amino acids at positions35, 189, 280, 284, 285, 334 or 373,said positions being defined with reference to SEQ ID NO: 2 and wherein the polypeptide has one or more modified properties as compared with a reference polypeptide having UGT activity. A polypeptide according to the disclosure may be used in a recombinant cell for the production of steviol or a steviol glycoside.