Beads as Transposome Carriers

Beads as Transposome Carriers

Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3′ portion co...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Fisher, Jeffrey S, Brodin, Jeffrey, Storms, Lena, Shen, Fei, Khurana, Tarun Kumar, Rosàs-Canyelles, Elisabet, Wu, Yir-Shyuan
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NANOTECHNOLOGY
PERFORMING OPERATIONS
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES
SPIRITS
TRANSPORTING
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Degradable polyester bead are described comprising a plurality of transposome complexes immobilized to the surface thereof, wherein each transposome complex comprises a transposase bound to a first polynucleotide and a second polynucleotide, wherein the first polynucleotide comprises a 3′ portion comprising a transposon end sequence and a tag, and the second polynucleotide comprises a 5′ portion that is complementary to and hybridized to the transposon end sequence, and wherein the polyester bead has a melting point of from 50° C. to 65° C. Flow cells and methods related to these polyester beads are described. Also described herein are compositions comprising a bead and at least one nanoparticle and methods of use of such compositions comprising transposome complexes immobilized to nanoparticles.