SWITCH OLIGONUCLEOTIDE

The invention relates to a set of oligonucleotide primers and their use in methods for amplifying and detecting oligonucleotides, detecting pathogens, or diagnosing infections such as SARS-CoV-2 and Covid-19. The primer set includes a switch oligonucleotide that is adapted to anneal to a forward or...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	LIM, Boon Chuan, CUI, Zhanfeng, YU, Yejiong, HSU, Chia-Chen, HUANG, Wei
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a set of oligonucleotide primers and their use in methods for amplifying and detecting oligonucleotides, detecting pathogens, or diagnosing infections such as SARS-CoV-2 and Covid-19. The primer set includes a switch oligonucleotide that is adapted to anneal to a forward or reverse primer of the set at temperatures that are below the temperature range for amplification of the DNA. The switch oligonucleotide prevents amplification from the complementary primer when the complementary primer is bound to the switch oligonucleotide. The invention also provides a method of reducing false positives in the detection of a target DNA or RNA sequence using loop-mediated isothermal amplification (LAMP) or reverse transcription loop-mediated isothermal amplification (RT-LAMP), by using such a switch oligonucleotide.