A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO

A HIGHLY ERROR-PRONE ORTHOGONAL DNA REPLICATION SYSTEM FOR TARGETED CONTINUOUS EVOLUTION IN VIVO

The invention provides compositions comprising highly error-prone polymerases and methods of using the polymerases for rapid evolution of a nucleic acid sequence within host cells. The invention further provides a versatile synthetic biology platform for manipulating DNA replication inside a cell. T...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Arzumanyan, Garri A, Liu, Chang C, Ravikumar, Arjun, Javanpour, Alex A
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The invention provides compositions comprising highly error-prone polymerases and methods of using the polymerases for rapid evolution of a nucleic acid sequence within host cells. The invention further provides a versatile synthetic biology platform for manipulating DNA replication inside a cell. The invention also provides a mutually orthogonal replication system for manipulating and tuning DNA replication of multiple nucleic acid molecules using the error-prone polymerases.