MULTI-CHANNEL EXTENDED DEPTH-OF-FIELD METHOD FOR AUTOMATED DIGITAL CYTOLOGY

A method for generating a color-faithful extended-depth-of-field (EDF) image from a color volume of 2D images acquired at different focal depths using a microscope. The method involves: generating a grayscale volume; applying invertible color-to-grayscale transformation to the volume; applying wavel...

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Hauptverfasser: BOUYSSOUX, Alexandre, FEZZANI, Riadh, OLIVO-MARIN, Jean-Christophe
Format: Patent
Sprache:eng
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Zusammenfassung:A method for generating a color-faithful extended-depth-of-field (EDF) image from a color volume of 2D images acquired at different focal depths using a microscope. The method involves: generating a grayscale volume; applying invertible color-to-grayscale transformation to the volume; applying wavelet transform to the grayscale volume to obtain a 3D wavelet-coefficient-matrix (WCM); selecting wavelet coefficients using a coefficient selection rule; generating a 2D-WCM and a 2D coefficient-map (CM); applying inverse transformation of the wavelet transform to the 2D-WCM to obtain a 2D grayscale EDF image; generating a 2D color-composite(CC) image; applying inverse transformation of the color-to-grayscale transformation to the 2D grayscale EDF image to obtain a 2D color EDF image; converting the 2D-CC image and the 2D color EDF image into a color space including chromaticity and intensity component(s); and concatenating, chromaticity component(s) of the 2D-CC image and intensity component(s) of the 2D color EDF image, to obtain a color-faithful EDF image.