WHOLE GENOME SGRNA LIBRARY CONSTRUCTING SYSTEM AND APPLICATION THEREOF

WHOLE GENOME SGRNA LIBRARY CONSTRUCTING SYSTEM AND APPLICATION THEREOF

Provided are a system for constructing a genome-wide sgRNA library and a use thereof. The system includes an input module, an sgRNA design module and an sgRNA filtering module. By constructing three modules in the system, optimizing details and processes in the modules, and adopting multiple design...

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Bibliographische Detailangaben
Hauptverfasser: Duan, Guangyou, Xu, Pengyang, Xu, Fengdan, Jin, Liang, Zhao, Wenyan, Ge, Yi
Format: Patent
Sprache:eng
Schlagworte:
INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTEDFOR SPECIFIC APPLICATION FIELDS
PHYSICS
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Beschreibung
Zusammenfassung:Provided are a system for constructing a genome-wide sgRNA library and a use thereof. The system includes an input module, an sgRNA design module and an sgRNA filtering module. By constructing three modules in the system, optimizing details and processes in the modules, and adopting multiple design criteria and screening principles, the genome-wide sgRNA library is finally constructed. The system and method are concise and efficient, and the obtained library has a high quality and good activity, and is convenient for applications in gene editing researches.